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membrane potential assay kit  (Beyotime)


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    Structured Review

    Beyotime membrane potential assay kit
    PDK1 inhibitor JX06 and gefitinib synergistically induced cell apoptosis in gefitinib-resistant lung cancer cells. (A) The protein expression levels of PDK were reduced upon the treatment of PDK1 inhibitor JX06 in PC-9 and PC-9/G cells. (B) B2B and PC-9/G cells were treated with different concentrations of JX06 for 48 h. The cell viabilities were determined by CCK-8. (C – E) The synergy effect between JX06 and gefitinib was determined and analyzed with CompuSyn software. (F) The apoptosis rates were analyzed with flow cytometry after the combined treatment of gefitinib and JX06. (G) The TUNEL <t>assay</t> was performed with the indicated treatment in PC-9/G cells. (H) The cells treated as described were stained with the JC-1 probe and detected using a fluorescence microscope. Red fluorescence indicates the aggregation form of JC-1, showing increased mitochondrial <t>membrane</t> <t>potential</t> (ΔΨm). Green fluorescence indicates the monomeric form of JC-1, which indicates reduced mitochondrial membrane potential (ΔΨm). Data were statistically analyzed with Student’s t -test, and values were shown as mean ± standard deviation. ∗ P < 0.05 and ∗∗ P < 0.01.
    Membrane Potential Assay Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 775 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/membrane+potential+assay+kit/Enhanced+mitochondrial+membrane+potential+assay+kit+with+JC-1/pmc13091346-69-18-25
    Average 99 stars, based on 775 article reviews
    membrane potential assay kit - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "PDK1 elevation was induced by epigenetic modifications of KDM3A and METTL16 to mediate TKI resistance and cancer development"

    Article Title: PDK1 elevation was induced by epigenetic modifications of KDM3A and METTL16 to mediate TKI resistance and cancer development

    Journal: Genes & Diseases

    doi: 10.1016/j.gendis.2025.101947

    PDK1 inhibitor JX06 and gefitinib synergistically induced cell apoptosis in gefitinib-resistant lung cancer cells. (A) The protein expression levels of PDK were reduced upon the treatment of PDK1 inhibitor JX06 in PC-9 and PC-9/G cells. (B) B2B and PC-9/G cells were treated with different concentrations of JX06 for 48 h. The cell viabilities were determined by CCK-8. (C – E) The synergy effect between JX06 and gefitinib was determined and analyzed with CompuSyn software. (F) The apoptosis rates were analyzed with flow cytometry after the combined treatment of gefitinib and JX06. (G) The TUNEL assay was performed with the indicated treatment in PC-9/G cells. (H) The cells treated as described were stained with the JC-1 probe and detected using a fluorescence microscope. Red fluorescence indicates the aggregation form of JC-1, showing increased mitochondrial membrane potential (ΔΨm). Green fluorescence indicates the monomeric form of JC-1, which indicates reduced mitochondrial membrane potential (ΔΨm). Data were statistically analyzed with Student’s t -test, and values were shown as mean ± standard deviation. ∗ P < 0.05 and ∗∗ P < 0.01.
    Figure Legend Snippet: PDK1 inhibitor JX06 and gefitinib synergistically induced cell apoptosis in gefitinib-resistant lung cancer cells. (A) The protein expression levels of PDK were reduced upon the treatment of PDK1 inhibitor JX06 in PC-9 and PC-9/G cells. (B) B2B and PC-9/G cells were treated with different concentrations of JX06 for 48 h. The cell viabilities were determined by CCK-8. (C – E) The synergy effect between JX06 and gefitinib was determined and analyzed with CompuSyn software. (F) The apoptosis rates were analyzed with flow cytometry after the combined treatment of gefitinib and JX06. (G) The TUNEL assay was performed with the indicated treatment in PC-9/G cells. (H) The cells treated as described were stained with the JC-1 probe and detected using a fluorescence microscope. Red fluorescence indicates the aggregation form of JC-1, showing increased mitochondrial membrane potential (ΔΨm). Green fluorescence indicates the monomeric form of JC-1, which indicates reduced mitochondrial membrane potential (ΔΨm). Data were statistically analyzed with Student’s t -test, and values were shown as mean ± standard deviation. ∗ P < 0.05 and ∗∗ P < 0.01.

    Techniques Used: Expressing, CCK-8 Assay, Software, Flow Cytometry, TUNEL Assay, Staining, Fluorescence, Microscopy, Membrane, Standard Deviation

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    Membrane:

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    Article Snippet: Cognitive impairment is the primary manifestation of neuroinflammation-related central nervous system diseases.. Intranasal administration is an effective method, bypassing the blood-brain barrier and delivering drugs to the brain.. Herein, we designed a biomimetic self-propelled nanomotor with an inflammation-targeting capacity.

    Article Title: Microplastics released from dental materials induce oral inflammatory bone resorption and apoptosis via mitochondrial dysfunction.
    Article Snippet: .. MMPs was measured using a JC-1 mitochondrial membrane potential assay kit (Beyotime, China) via immunofluorescence. .. Cells were rinsed with serum-free medium and incubated with JC-1 working solution at 37◦C for 20 min. After rinsing with JC-1 dilution buffer, images were immediately captured using an inverted fluorescence microscope (Leica Microsystems, Germany) under consistent parameters for all samples.

    Article Title: Exportin 1 Inhibitor Combined With Venetoclax Induces Apoptosis in Myelodysplastic Syndrome by Mitochondria-Induced Apoptosis Pathway.
    Article Snippet: All samples were acquired with a DxFlex system (Beckman Coulter). .. We detected loss of MOMP (ΔΨm) using the Enhanced mitochondrial membrane potential assay kit with JC- 1 (Beyotime, Nanjing, China, C2003S). ..

    Article Title: PDK1 elevation was induced by epigenetic modifications of KDM3A and METTL16 to mediate TKI resistance and cancer development
    Article Snippet: .. After different treatments for 48 h, the mitochondrial membrane potential of cells was detected with the enhanced mitochondrial membrane potential assay kit using JC-1 probe (Beyotime, Jiangsu, China), and the fluorescence was analyzed with a fluorescence microscope. ..

    Article Title: OTUB1/CDYL axis-mediated epigenetic repression of SOX18 facilitates lung cancer progression by inhibiting FDX1-dependent cuproptosis.
    Article Snippet: Lung cancer persists as a major contributor to global cancer-related mortality, with metastasis, recurrence, and therapy resistance posing substantial barriers to effective disease management.. CDYL has gained recognition as an epigenetic co-repressor involved in multiple dimensions of oncogenesis.. However, its precise mechanistic contributions to non-small cell lung cancer (NSCLC) pathogenesis remain inadequately characterized.

    Article Title: FAP-targeting peptide-directed nanoprobes enable tumor microenvironment-activatable MR/NIRF imaging of breast cancer primary tumor and lung metastases
    Article Snippet: .. Following this, the cells were stained using the enhanced mitochondrial membrane potential assay kit with JC-1 (Beyotime Ltd, China) according to the manufacturer's instructions. .. Finally, the cells were observed under a fluorescence microscope (OLYMPUS, Japan).

    Article Title: Zinc Overload in Microvessels Contributes to Blood-Brain Barrier Disruption by Activating the JAK2 Pathway After Cerebral Ischemia/Reperfusion.
    Article Snippet: .. Mitochondrial membrane potential (ΔΨm) was assessed using a JC- 1 mitochondrial membrane potential assay kit (Beyotime, China). ..

    Article Title: A novel L-shaped ortho-quinone analog and 2-Deoxy-D-Glucose a synergistic approach to inhibit hepatocellular carcinoma cell proliferation.
    Article Snippet: .. 2.8 Detection of mitochondrial membrane potential change We used the enhanced mitochondrial membrane potential assay kit with JC-1 (Beyotime) to detect the mitochondrial membrane potential change in cell. ..

    CCK-8 Assay:

    Article Title: A 3-N nose-to-brain urolithin a nanomotor targeting microglial mitophagy in neuroinflammation.
    Article Snippet: Cognitive impairment is the primary manifestation of neuroinflammation-related central nervous system diseases.. Intranasal administration is an effective method, bypassing the blood-brain barrier and delivering drugs to the brain.. Herein, we designed a biomimetic self-propelled nanomotor with an inflammation-targeting capacity.

    Immunofluorescence:

    Article Title: Microplastics released from dental materials induce oral inflammatory bone resorption and apoptosis via mitochondrial dysfunction.
    Article Snippet: .. MMPs was measured using a JC-1 mitochondrial membrane potential assay kit (Beyotime, China) via immunofluorescence. .. Cells were rinsed with serum-free medium and incubated with JC-1 working solution at 37◦C for 20 min. After rinsing with JC-1 dilution buffer, images were immediately captured using an inverted fluorescence microscope (Leica Microsystems, Germany) under consistent parameters for all samples.

    Fluorescence:

    Article Title: PDK1 elevation was induced by epigenetic modifications of KDM3A and METTL16 to mediate TKI resistance and cancer development
    Article Snippet: .. After different treatments for 48 h, the mitochondrial membrane potential of cells was detected with the enhanced mitochondrial membrane potential assay kit using JC-1 probe (Beyotime, Jiangsu, China), and the fluorescence was analyzed with a fluorescence microscope. ..

    Microscopy:

    Article Title: PDK1 elevation was induced by epigenetic modifications of KDM3A and METTL16 to mediate TKI resistance and cancer development
    Article Snippet: .. After different treatments for 48 h, the mitochondrial membrane potential of cells was detected with the enhanced mitochondrial membrane potential assay kit using JC-1 probe (Beyotime, Jiangsu, China), and the fluorescence was analyzed with a fluorescence microscope. ..

    Staining:

    Article Title: FAP-targeting peptide-directed nanoprobes enable tumor microenvironment-activatable MR/NIRF imaging of breast cancer primary tumor and lung metastases
    Article Snippet: .. Following this, the cells were stained using the enhanced mitochondrial membrane potential assay kit with JC-1 (Beyotime Ltd, China) according to the manufacturer's instructions. .. Finally, the cells were observed under a fluorescence microscope (OLYMPUS, Japan).



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    Image Search Results


    MCU regulates mitophagy and mitochondrial function. A. GSEA analysis showed that MCU-associated genes were significantly enriched in the “autophagy of mitochondrion” and “positive regulation of autophagy of mitochondrion” pathways; B. Western blot analysis was performed to detect the expression of mitophagy-related proteins, including PINK1, PRKN, and LC3B, in H1975 and PC9 cells; C, D. Immunofluorescence analysis was used to examine the colocalization of mitochondria and LC3B in H1975 and PC9 cells; E, F. JC-1 staining combined with flow cytometry was performed to assess changes in mitochondrial membrane potential in H1975 and PC9 cells; G, H. Flow cytometry was used to detect intracellular ROS levels in H1975 and PC9 cells. Data are presented as the mean ± SD. Statistical analysis was performed using two-sided t-tests. **P < 0.01; ***P < 0.001; ****P < 0.0001.

    Journal: American Journal of Cancer Research

    Article Title: Mitochondrial calcium uniporter as a biomarker in lung adenocarcinoma: regulation of the immune microenvironment, apoptosis, cell cycle and mitophagy

    doi: 10.62347/PGQK3358

    Figure Lengend Snippet: MCU regulates mitophagy and mitochondrial function. A. GSEA analysis showed that MCU-associated genes were significantly enriched in the “autophagy of mitochondrion” and “positive regulation of autophagy of mitochondrion” pathways; B. Western blot analysis was performed to detect the expression of mitophagy-related proteins, including PINK1, PRKN, and LC3B, in H1975 and PC9 cells; C, D. Immunofluorescence analysis was used to examine the colocalization of mitochondria and LC3B in H1975 and PC9 cells; E, F. JC-1 staining combined with flow cytometry was performed to assess changes in mitochondrial membrane potential in H1975 and PC9 cells; G, H. Flow cytometry was used to detect intracellular ROS levels in H1975 and PC9 cells. Data are presented as the mean ± SD. Statistical analysis was performed using two-sided t-tests. **P < 0.01; ***P < 0.001; ****P < 0.0001.

    Article Snippet: JC-1 assay kit (MedChemExpress, New Jersey, USA) is to be used for the mitochondrial membrane potential experiment.

    Techniques: Western Blot, Expressing, Immunofluorescence, Staining, Flow Cytometry, Membrane

    PDK1 inhibitor JX06 and gefitinib synergistically induced cell apoptosis in gefitinib-resistant lung cancer cells. (A) The protein expression levels of PDK were reduced upon the treatment of PDK1 inhibitor JX06 in PC-9 and PC-9/G cells. (B) B2B and PC-9/G cells were treated with different concentrations of JX06 for 48 h. The cell viabilities were determined by CCK-8. (C – E) The synergy effect between JX06 and gefitinib was determined and analyzed with CompuSyn software. (F) The apoptosis rates were analyzed with flow cytometry after the combined treatment of gefitinib and JX06. (G) The TUNEL assay was performed with the indicated treatment in PC-9/G cells. (H) The cells treated as described were stained with the JC-1 probe and detected using a fluorescence microscope. Red fluorescence indicates the aggregation form of JC-1, showing increased mitochondrial membrane potential (ΔΨm). Green fluorescence indicates the monomeric form of JC-1, which indicates reduced mitochondrial membrane potential (ΔΨm). Data were statistically analyzed with Student’s t -test, and values were shown as mean ± standard deviation. ∗ P < 0.05 and ∗∗ P < 0.01.

    Journal: Genes & Diseases

    Article Title: PDK1 elevation was induced by epigenetic modifications of KDM3A and METTL16 to mediate TKI resistance and cancer development

    doi: 10.1016/j.gendis.2025.101947

    Figure Lengend Snippet: PDK1 inhibitor JX06 and gefitinib synergistically induced cell apoptosis in gefitinib-resistant lung cancer cells. (A) The protein expression levels of PDK were reduced upon the treatment of PDK1 inhibitor JX06 in PC-9 and PC-9/G cells. (B) B2B and PC-9/G cells were treated with different concentrations of JX06 for 48 h. The cell viabilities were determined by CCK-8. (C – E) The synergy effect between JX06 and gefitinib was determined and analyzed with CompuSyn software. (F) The apoptosis rates were analyzed with flow cytometry after the combined treatment of gefitinib and JX06. (G) The TUNEL assay was performed with the indicated treatment in PC-9/G cells. (H) The cells treated as described were stained with the JC-1 probe and detected using a fluorescence microscope. Red fluorescence indicates the aggregation form of JC-1, showing increased mitochondrial membrane potential (ΔΨm). Green fluorescence indicates the monomeric form of JC-1, which indicates reduced mitochondrial membrane potential (ΔΨm). Data were statistically analyzed with Student’s t -test, and values were shown as mean ± standard deviation. ∗ P < 0.05 and ∗∗ P < 0.01.

    Article Snippet: After different treatments for 48 h, the mitochondrial membrane potential of cells was detected with the enhanced mitochondrial membrane potential assay kit using JC-1 probe (Beyotime, Jiangsu, China), and the fluorescence was analyzed with a fluorescence microscope.

    Techniques: Expressing, CCK-8 Assay, Software, Flow Cytometry, TUNEL Assay, Staining, Fluorescence, Microscopy, Membrane, Standard Deviation